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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

HSP70 | salmonid heat shock protein 70

AS05 061 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmo sp. (salmon), Salvelinus fontinalis (brook trout)

HSP70 | salmonid heat shock protein 70 in the group Human/Animal Antibodies / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 061)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to fish related targets

Secondary antibodies

Additional information

The antibody is very specific for salmonid inducible form - hsp70. It does not cross-react with hsc70. It does not detect hsp70 from other species.

application information
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

70 kDa

Confirmed reactivity Rainbow trout (Oncorhynchus mykiss), Brook trout gills (Salvelinus fontinalis)
Predicted reactivity

Salmo salar (Atlantic salmon)

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Kelly et al. (2017). Acclimation capacity of the cardiac HSP70 and HSP90 response to thermal stress in lake trout (Salvelinus namaycush), a stenothermal ice-age relict. Comp Biochem Physiol B Biochem Mol Biol. 2017 Dec 10. pii: S1096-4959(17)30191-4. doi: 10.1016/j.cbpb.2017.12.002.
Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.
Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: β-adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com