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AGO set
Tag Antibody Set 

HSP70 | salmonid heat shock protein 70

AS05 061 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmo sp. (salmon), Salvelinus fontinalis (brook trout)

HSP70 | salmonid heat shock protein 70 in the group Antibodies for Human/Animal  / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 061)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Rainbow trout (Oncorhynchus mykiss), Brook trout gills (Salvelinus fontinalis)
Predicted reactivity

Salmo salar (Atlantic salmon)

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

The antibody is very specific for salmonid inducible form - hsp70. It does not cross-react with hsc70. It does not detect hsp70 from other species.

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Background

Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Product citations

Selected references Kelly et al. (2017). Acclimation capacity of the cardiac HSP70 and HSP90 response to thermal stress in lake trout (Salvelinus namaycush), a stenothermal ice-age relict. Comp Biochem Physiol B Biochem Mol Biol. 2017 Dec 10. pii: S1096-4959(17)30191-4. doi: 10.1016/j.cbpb.2017.12.002.
Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.
Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: β-adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.

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