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AGO set
Tag Antibody Set 

HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70 (serum)

AS05 083 |  Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: [global abtibody] for HSP70 and HSC70 in fish, mammals and fungi

HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70 (serum) in the group Antibodies for Human/Animal  / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 083)

PRODUCT INFORMATION IN PDF

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342 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (IP), 1 : 1000-1: 5000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Catostomus commersonii, Gloeophyllum protractum, Gloeophyllum sepiarium, G. carbonarium, Junghunia luteoalba, Oligoporus sericiomollis, Phlebia cornea
Predicted reactivity Bovine, Drosophila melanogaster, Hen, Mouse, Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-HSP70 global antibody

10 µg of total protein from (1) killi fish muscle, (2) bovine muscle, (3) chicken muscle, (4) rat liver, extracted with Protein Extration Buffer, PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked in 5 % non-fat milk  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Agrisera anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 5 min.

Additional information

Additional information

For detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371.

This antibody is recognizing both, the inducible and the constitutive Hsp70.

Related products

Related products AS05 083A | Anti-HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70, Affinity purified, rabbit antibodies

Secondary antibodies

Background

Background

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1,   HSP70-1/HSP70-2,  Heat shock 70 kDa protein 8

Product citations

Selected references MacLellan et al. (2015). Chaperone roles for TMAO and HSP70 during hyposmotic stress in the spiny dogfish shark (Squalus acanthias). J Comp Physiol B. 2015 Jun 7.
Bessemer et al. (2014). Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology. 2014 Nov 27:1-10.
Gorovits et al. (2013). Recruitment of the host plant heat shock protein 70 by tomato yellow leaf curl virus coat protein is required for virus infection. PLoS Once, July 23;8(7).

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