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AGO set
Tag Antibody Set 

HSP90 | Heat shock protein 90

AS05 063  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmon, Human

HSP90 | Heat shock protein 90 in the group Antibodies for Human/Animal  / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 063)

PRODUCT INFORMATION IN PDF

Qty: 
343 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

84-86 kDa

Reactivity

Confirmed reactivity Human, Salmon
Predicted reactivity Fishes, Hen, Mammals
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

 

Additional information

in salmonid fish a cross-reactive band at approximately 40 kDa is observed; antibody will also detect a human recombinant HSP90 protein

Antibody is reacting strongly with recombinant HSP90 from HELA cells. 

Related products

Related products

AS05 061 | anti-salmonid HSP70

AS05 061A | anti-salmonid HSP70, suitable for immunoprecipitation

AS05 062 | anti-salmonid HSC70

AS05 083A | anti-HSP70/HSC70 global antibody, affinity purified

Secondary antibodies

Background

Background

HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84

Product citations

Selected references Kelly et al. (2017). Acclimation capacity of the cardiac HSP70 and HSP90 response to thermal stress in lake trout (Salvelinus namaycush), a stenothermal ice-age relict. Comp Biochem Physiol B Biochem Mol Biol. 2017 Dec 10. pii: S1096-4959(17)30191-4. doi: 10.1016/j.cbpb.2017.12.002.
Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.
Liu et al. (2014). Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays. J. Agronomy and Crop Science, Article first published online: 1 APR 2014, DOI: 10.1111/jac.12058.
Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.

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