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BDNF | Brain-derived neurotrophic factor

AS13 2691 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat, Mouse, Human

BDNF | Brain-derived neurotrophic factor in the group Human/Animal Antibodies / Human Proteins / Amyloid disorders / Parkinson's disease at Agrisera AB (Antibodies for research) (AS13 2691)

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Background

BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. It promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Binds to NTRK2/TRKB.  It is converted into mature BDNF by plasmin (PLG). Belongs to the NGF-beta family.

Immunogen

KLH-conjugated synthetic peptide HSDPARRGEL. The BDNF protein sequence is highly conserved amongst primates.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products AS16 3242 | BDNF | Brain Derived Neurotrophic Factor (affinity purified)
Additional information
application information
Recommended dilution 1 : 1000-1 : 2000 (ELISA), (IHC), (WB)
Expected | apparent MW
Confirmed reactivity Human, Mouse, Rat
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer), depending upon sample type (human serum, cell lysate, tissue homogenate.

This antibody is also recognizing BDNF in non denaturing conditions.

Cross reaction with mouse NGF recombinant human NT3 and NT4/5 is lower than 0.1 % as confirmed by dot blot analysis.
Selected references








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