GDE / AGL | Glycogen debranching enzyme

381 €

AS09 454 | Clonality:Polyclonal | Host:Rabbit | Reactivity:Human, Mouse, Rabbit, Rat


23 st
Item No:
AS09 454

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product information

Synonymes:Glycogen debrancher, 4-alpha-glucanotransferase (EC=, Oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-1,6-glucosidase (EC=, Dextrin 6-alpha-D-glucosidase,amylo-1, 6-glucosidase, 4-alpha-glucanotransferase


KLH-conjugated peptide derived from the sequence of human glycogen debranching enzyme P35573

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Related products

AS09 455 | anti-glycogen phosphorylase

Secondary antibodies

Additional information

This antibody can detect GDE in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GDE.

Antibody will work as an excellent marker for glycogen. In tissues with no glycogen extression there will be no signal using this antibody. 

application information
Recommended dilution 5 ĩg (IP), 1 : 2000 (WB)
Expected | apparent MW

175 kDa

Confirmed reactivity Human, Mouse, Rabbit,Rat
Predicted reactivity Dog
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Samples used to test this antibody were: muscle and liver homogenates, purified glycogen. This antiboy will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes.
Selected references Oldenburg et al. (2016). CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer. 2016 Sep 5;16:713. doi: 10.1186/s12885-016-2756-5.
Pagliarani et al. (2014). Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Aug 1. pii: S0925-4439(14)00250-6. doi: 10.1016/j.bbadis.2014.07.029.

application example

(1) rat liver homogenate (50 µg of total protein), (2) GDE IP from 500 µg rat liver homogenate (3) GDE IP from 10 µg purified rat liver glycogen (see Parker et al, BBRC 2007) were resuspended into SDS PAGE sample buffer, boiled, electrophoresed on a pre-cast 4-15% gradient gel (Invitrogen, CA, USA) and transferred to PVDF membrane. Following blocking in 5% milk / PBST, membranes were probed with GDE antibodies (diluted 1/2000) for 1h at room temperature with rocking, followed by washing in 3 times for 5 min in PBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Protein-G coupled to horse radish peroxidase conjugated – Bio-Rad) diluted 1:3000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL (Invitrogen, CA, USA). Images were detected using the Fuji LAS-3000 system.

To obtain GDE-bound immunoprecipitates, 5 µg GDE antibody was incubated with 500 µg rat liver homogenate or 10 ug purified rat liver glycogen together with 1 ml IP wash buffer (50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1% NP-40, 1 mM EGTA, 10 mM NaPPi and 100 mM NaF) and incubated at 4°C with mixing for 90 minutes. 100µ l, 20% Protein-A Sepharose was then added for a further 30 minutes at 4°C with mixing. Immunoprecipitates were collected by centrifugation at 7,000 rpm for 1 minutes and washed three times with IP wash buffer followed by resuspension into SDS PAGE sample buffer, boiling and electrophoresis as above.


western blot using anti-GDE antibodies

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