mLrig3-207 | Leucine-rich repeats and immunoglobulin-like domains protein 3

AS14 2789  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: mice

mLrig3-207 | Leucine-rich repeats and immunoglobulin-like domains protein 3 in the group Human/Animal Antibodies / Human Proteins / Other Human proteins at Agrisera AB (Antibodies for research) (AS14 2789)


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product information

Lrig3 (Leucine-rich repeats and immunoglobulin-like domains protein 3), involved in craniofacial and inner ear morphogenesis during embryonic development. 


KLH-conjugated synthetic peptide derived from mice Lrig3 protein, UniProt: Q6P1C6

Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution 1 : 500 (3 µg/ml)
Expected | apparent MW

122 | 140 kDa

Confirmed reactivity Mus musculus
Predicted reactivity Bos taurus, Sus scrofa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody reactivity was confirmed on skin tissue lysates from wildtype vs. Lrig3 knockout mice.
Selected references Hellstrom et al. (2016). Cardiac hypertrophy and decreased high-density lipoprotein cholesterol in Lrig3-deficient mice. Am J Physiol Regul Integr Comp Physiol. 2016 Mar 23:ajpregu.00309.2015. doi: 10.1152/ajpregu.00309.2015.

Thirty µg of total protein from mouse skin tissue (wild-type and Lrig3 knock-out mice) extracted with modified RIPA buffer were separated by electrophoresis on a 3 – 8% Tris-acetate NuPAGE gradient gel and blotted to a PVDF membrane. Blots were blocked with 5% nonfat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary Lrig3 antibody, upper part, at a dilution of 1:500 for 1h at RT with agitation (actin antibody, lower part). The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from GE Healthcare) diluted to 1:20,000 for 1h at RT with agitation. The blot was washed as above and developed with ECL according to the manufacturer’s instructions.

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