SOD1 aa 131-153 | superoxide dismutase 1, soluble

AS09 540 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

SOD1 aa 131-153 | superoxide dismutase 1, soluble  in the group Human/Animal Antibodies / Human Proteins / Oxidative stress at Agrisera AB (Antibodies for research) (AS09 540)


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product information

SOD1 [Cu-Zn] (EC= is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic


KLH-conjugated synthetic peptide, amino acids 131-153 derived from human SOD1 sequence P00441

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution 1 : 75 000 (WB)
Expected | apparent MW

15.9 kDa

Confirmed reactivity Human
Predicted reactivity Atlantic Salmon, Bovine, Chimpanzee, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Peptide used to elicit this antibody is not conserved in SOD2,3 and 4.

Selected references Kiskinis et al. (2014). Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1. Cell Stem Cell. 2014 Apr 2. pii: S1934-5909(14)00098-8. doi: 10.1016/j.stem.2014.03.004.

application example 



 10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 540) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.


 western blot using anti-SOD1 antibodieswestern blot using anti-SOD1 antibodies aa 135-153

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