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Gly | glycine

AS09 593  |  Clonality: Polyclonal  |  Host: Rabbit

Gly | glycine in the group Human/Animal Antibodies / Neurosteroids/Neurobiology at Agrisera AB (Antibodies for research) (AS09 593)

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product information
Background

Glycine (Gly) is a smallest of amino acids found in proteins. 

Immunogen

Synthetic glycine conjugated to protein carrier.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity

1 mg

Reconstitution For reconstitution add 500 µl of sterile water and 500 µl of glycerol.
Storage Store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Related products

Secondary antibodies

Additional information
Compounds Cross-reactivity ratio (a)
   
Glycine-G-PC* 1
β−Alanine-G-PC 1/100
GABA-G-PC 1/500
Taurine-G-PC 1/1 000
L.Aspartate-G-PC 1/20 000
L.Glutamate-G-PC 1/>20 000
Free Glycine 1/>50 000

G- glutaraldehyde, (a) Glycine-G-PC concentration/unconjugated or conjugated amio acid concentration at half displacement

application information
Recommended dilution 1 : 1000-1 : 5 000 (ICC)
Expected | apparent MW
Confirmed reactivity glycine
Predicted reactivity Glycine
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information For example of immunocytochemistry protocol check here.total IgG was purified by ammonium sulfate precipitation.
Selected references Rubio & Juiz (2004). Differential distribution of synaptic endings containing glutamate, glycine, and GABA in the rat dorsal cochlear nucleus. J. Comp. Neurol. 477: 253-272.
Van Zundert et al. (2000). Effects of chronic ethanol treatment on gamma-aminobutyric acid(A) and glycine receptors in mouse glycinergic spinal neurons. J. Pharmacol. Exp. ther. 1:423-429.


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