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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

THP | Allopregnanolone steroid standard

AS04 041S  |  GMP manufactured steroid standard

THP | Allopregnanolone steroid standard in the group Human/Animal Antibodies / Neurosteroids/Neurobiology at Agrisera AB (Antibodies for research) (AS04 041S)

PRODUCT INFORMATION IN PDF

Qty: 
245 €

Info: Add review
product information
Background

Allopregnanolone, (3alpha-hydroxy-20-oxo-5alphapregnan-11alpha-yl-carboxymethylether), also known as 3alpha,5alpha-tetrahydroprogesterone or THP, a neuroactive steroid secreted by the mammalian ovary, exerts its anesthetic, anxiolytic, and sedative/hypnotic effects through potentiation of GABA A receptors. It is produced in the human corpus luteum and their release is stimulated by trophic hormone.

Host
Clonality
Clone
Purity
Format Lyophilized
Quantity 10 mg
Reconstitution
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Protect from light. Can be stored for 1 month at 4°C in a sealed tube or flask.
Tested applications Radioimmunoassay (RIA)
Related products

AS04 041 | anti-allopregnanolone antibody

Additional information

Allopregnanolone standard is made under GMP conditions and thus has the purity standards required for human usage in clinical trials. Produced by Umecrine AB.

application information
Recommended dilution
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information The lyophilized powder of this standard is not soluble in water.

Standard dissolving instruction:
  • Use ethanol (99.5 %) or methanol to prepare a concentrated (stock) solution of THP standard. This solution can be stored for longer time in a sealed tube.
  • Working solution can be used daily but needs to be changed after one month.
  • Working solution is used to prepare standard points 0-200 pg. It needs to be dried under nitrogen before it is diluted in water based soltion, otherwise the steroid will oxidase.
  • Following dyring, water based solution can be added to this standard.
Selected references Lee at al. (2014). Progesterone and allopregnanolone improve stroke outcome in male mice via distinct mechanisms but neither promotes neurogenesis. J of Neurochemistry, DOI: 10.1111/jnc.12990.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com