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AGO set
Tag Antibody Set 

DS5a | Drosophila 26S proteasome subunit Rpn10

AS01 012  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Drosophila melanogaster

DS5a | Drosophila 26S proteasome subunit Rpn10 in the group Antibodies for Human/Animal  / Drosophila melanogaster at Agrisera AB (Antibodies for research) (AS01 012)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Protocols Add review

Product Information

Immunogen

Drosophila mealnogaster 26S Proteasome subunit S5a also known as Rpn10, p54. Antigen sequence is also conserved in Arabidopsis thaliana AT4G38630

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

42.6 | 54 kDa (Drosophila), 40 kDa (A. thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Drosophila melanogaster
Predicted reactivity Bovine, Mouse, Pig, Salmon, Rat,
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-RPN10 antibody

3 µg of total protein from  Arabidopis thaliana leaves  extracted with CelLytic P (Sigma)  were separated on 10  % SDS-PAGE and blotted overnight at 30V to a nitrocellulose membrane. Blots were blocked with 1% western blocking reagent (Roche) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (DS5a) at a dilution of 1:2000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, and then washed twice for 10 min with TBS-T at RT with agitation. Subsequently, the blot was washed twice with 0,5% western blocking reagent for 10 min each. Blot was incubated in secondary antibody (Goat anti-rabbit IgG (H&L) HRP conjugated, AS09 602 from Agrisera) diluted to 1:50 000 in 0,5% western blocking reagent for 1h at RT with agitation. The blot was washed 4 times with TBS-T buffer. Detection of HRP was performed with ECL according to the manufacturer's instructions. Exposure time was 2 seconds. Position of protein on gel corresponds to expected size - 40 kDa.

Courtesy Dr. Jozefus Schippers, Max Planck Institute, Germany

Additional information

Related products

Related products

ASA01 019 | human 26S proteasome subunit

Background

Background

Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. The subunit of the proteasome, S5a, was identified to bind to polyubiquitin in vitro and thus proposed to act as a substrate recognition component.

Product citations

Selected references Nguyen at al (2012). Anupstreamregulator of the26Sproteasomemodulatesorgansize in Arabidopsisthaliana. Plant J. Dec 17.
Lundgren et al. (2003). Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13. Mol Cel Biol 23:5320-5330.

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