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Arabinogalactan-protein, AGP (monoclonal, clone LM2)

AS18 4211  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
Arabinogalactan-protein, AGP (monoclonal, clone LM2) in the group Antibodies for Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4211)

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Product Information

Immunogen Polysaccharide Arabinogalactan-protein (AGP)
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Format Cell culture supernatamt
Quantity 5 ml
Storage  Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide.

This antibody is made to rice arabinogalactan-proteins (AGPs) and it recognizes a carbohydrate epitope containing B-linked glucuronic acid. 
In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-O-methyl-B-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-B-D-GlcA.

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Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.

Product citations

Selected references Stacey et al. (1990). Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L Planta. 1990 Jan;180(2):285-92.doi: 10.1007/BF00194009.
Knox et al.(1991). Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991 ov;1(3):317-326.doi: 10.1046/j.1365-313X.1991.t01-9-00999.x.

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