showForm Anti-AtArf2 | ADP ribosylation factor 2 antibodies

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AtArf2 | ADP ribosylation factor 2

AS09 476 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AtArf2 | ADP ribosylation factor 2 in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 476)

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478
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

synthetic peptide derived from Arabidopsis thaliana  Q9SRC3

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 ĩl
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

20.5 | 29 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Arf antibodies (AS09 476, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

  western blot detection using anti-Arf antibodies

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Related products

Background

Background

ADP ribosylation factor 2 is a GTP-binding protein which is involved in protein trafficking. ArfAlternative name: ARF1-like protein U5

Product citations

Selected references

to be added when available