ATG5 | Autophagy protein 5 (human)

AS13 2695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

Product Information

Immunogen A synthetic peptide corresponding to the C-terminal of human ATG-5L, No immunogenic carrier protein was conjugated to the immunogen, Instead, Adjukine B has been used to enhance the immune response,

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 200-1 : 1000 (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Human

Not reactive in Arabidopsis thaliana

Application examples

Application examples Application example

Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.

Additional information

Background

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Product citations

Related products: ATG5 | Autophagy protein 5 (human)

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

Goat anti-Rabbit IgG (H&L), DyLight® 650 conjugated, min, cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins

AS12 2335 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

233 €

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	A synthetic peptide corresponding to the C-terminal of human ATG-5L, No immunogenic carrier protein was conjugated to the immunogen, Instead, Adjukine B has been used to enhance the immune response,
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 200-1 : 1000 (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
tested applications:	Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Picture (footer):	Application example Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.
Confirmed reactivity:	Human
not reactive in:	Arabidopsis thaliana
background:	FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.