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ATG5 | Autophagy protein 5 (human)

AS13 2695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

ATG5 | Autophagy protein 5 (human) in the group Antibodies for Human/Animal  / Human Proteins / Developmental biology at Agrisera AB (Antibodies for research) (AS13 2695)

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Product Information

Immunogen

A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B  has been used to enhance the immune response.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

After reconstitution keep aliquots at -20ºC for a higher stability, and at  4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 200-1 : 1000 (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Human
Not reactive in Arabidopsis thaliana

Application examples

Application examples Application example




Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.




Additional information

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Background

Background

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

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