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ATG5 | Autophagy protein 5 (human)
AS13 2695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
- Product Info
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Immunogen: A synthetic peptide corresponding to the C-terminal of human ATG-5L, No immunogenic carrier protein was conjugated to the immunogen, Instead, Adjukine B has been used to enhance the immune response, Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Tested applications: Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 200-1 : 1000 (IF), (IHC), (WB) - Reactivity
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Confirmed reactivity: Human Not reactive in: Arabidopsis thaliana - Application Examples
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Application example 
Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.
- Background
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Background: FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right
AS12 2335 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)