IKP

ATG5 | Autophagy protein 5 (human)

Product no: AS13 2695

AS13 2695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

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  • Product Info
  • Immunogen: A synthetic peptide corresponding to the C-terminal of human ATG-5L, No immunogenic carrier protein was conjugated to the immunogen, Instead, Adjukine B has been used to enhance the immune response,
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: After reconstitution keep aliquots at -20C for a higher stability. and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
    Tested applications: Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 200-1 : 1000 (IF), (IHC), (WB)
  • Reactivity
  • Confirmed reactivity: Human
    Not reactive in: Arabidopsis thaliana
  • Application Examples
  • Application example




    Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.




  • Background
  • Background:

    FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

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