ATG5 | Autophagy protein 5

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  ATG5 | Autophagy protein 5 (human)
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      <p><strong>AS13 2695 </strong>| Clonality:<strong> Polyclonal</strong> | Host: <strong>Rabbit</strong> | Reactivity: <strong>Human</strong></p>  
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          <span>Immunogen</span>
          <span><p>A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B  has been used to enhance the immune response.</p></span>
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          <span>Host</span>
          <span>Rabbit</span>
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          <span>Clonality</span>
          <span>Polyclonal</span>
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          <span>Purity</span>
          <span>Serum</span>
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          <span>Format</span>
          <span>Lyophilized</span>
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          <span>Quantity</span>
          <span>100 µl</span>
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          <span>Reconstitution</span>
          <span>For reconstitution add 100 µl of sterile water.</span>
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          <span>Storage</span>
          <span><p>After reconstitution keep aliquots at -20ºC for a higher stability, and at  4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.</p></span>
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          <span>Tested applications</span>
          <span>Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)</span>
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          <span>Recommended dilution</span>
          <span>1 : 200-1 : 1000 (IF), (IHC), (WB)</span>
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      <h3>Reactivity</h3>
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          <span>Confirmed reactivity</span>
          <span>Human</span>
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          <span>Not reactive in</span>
          <span><em>Arabidopsis thaliana</em></span>
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              <span>Application examples</span>
              <span><strong>Application example<br /><br /><br /><img src="https://www.agrisera.com/dokument/bibliotek/R-111-100_Rb-anti_APG5-Rb4.jpg" width="150" height="120" /><br /><br /></strong>Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.<strong><br /></strong><br /><br /><br /><br /></span>
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       <h3>Background</h3>
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              <span>Background</span>
              <span><p>FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.</p></span>
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