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ATG7 | Autophagy-related protein 7 (human)

AS13 2699 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Rat

ATG7 | Autophagy-related protein 7 (human) in the group Antibodies for Human/Animal  / Human Proteins / Developmental biology at Agrisera AB (Antibodies for research) (AS13 2699)

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Product Information

Immunogen

A synthetic peptide corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived  from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

After reconstitution keep aliquots at -20ºC for a higher stability, and at  4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution 1 : 200-1 : 3000 (IF), (IHC)

Reactivity

Confirmed reactivity Human, Rat

Application examples

Application examples Application example





Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (AS13 2699) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.


Additional information

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Background

Background

 Autophagy-related protein 7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1. Formation of the final GABARAPL1-PE conjugate is essential for autophagy.

Product citations

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