ATG7 | Autophagy-related protein 7 (human)
AS13 2699 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Rat
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Product Information
Immunogen
A synthetic peptide corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen, The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S, cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog),
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
After reconstitution keep aliquots at -20šC for a higher stability. and at 4šC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Tested applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution
1 : 200-1 : 3000 (IF), (IHC)
Reactivity
Confirmed reactivity
Human, Rat
Application examples
Application examples
Application example
Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (AS13 2699) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (AS13 2699) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Additional information
Background
Background
Autophagy-related protein 7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1. Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
Product citations
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