showForm Anti-AtMRP1, AtABCC1 | ABC transporter C family member 1 antibodies

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MRP1, ABCC1 | ABC transporter C family member 1

AS09 472 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

MRP1, ABCC1 | ABC transporter C family member 1 in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 472)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MRP1,Q9C8G9, At1g30400

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

181.9 |180 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Oryza sativa, Populus balsamifera, Physcomitrella patens, Triticum aestivum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-AtABCC1 antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP4 antibodies (AS09 472, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Related products

Background

Background

MRP1 (EC= 3.6.3.44) is a pump for glutathione S-conjugates. Alternative names: ABC transporter ABCC.1, multidrug resistance-associated protein 1, glutathione S-conjugate-transporting ATPase 1, ATP-energized glutathione S-conjugate pump 1

Product citations

Selected references Geisler et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.

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