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ATPase | ATP synthase, whole enzyme

AS08 370  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: C. reinhardtii, E. opiuroides, S. oleracea, P. sativum, P. vaginatum

ATPase | ATP synthase, whole enzyme in the group Antibodies for Plant/Algal  / Bioenergetics at Agrisera AB (Antibodies for research) (AS08 370)

DATA SHEET IN PDF

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363
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Purified native protein from spinach

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 50 µl of sterile water. To get the best possible dissolution please keep it at +4°C ON.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

38-58 kDa

Reactivity

Confirmed reactivity Subunits alpha, beta, gamma, epsilon of Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Pisum sativum, perennial grasses: Eremochloa ophiuriudes, Paspalum vaginatum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

The prominent reaction is to a doublet around 55-58 kDa which is alpha and beta and weaker reaction to gamma subunit which is around 38 kDa.

Related products

Related products

AS08 304 | Anti-ATP synthase subunit alpha, rabbit antibodies

AS03 030 | Anti-ATP synthase subunit beta, hen antibodies

AS08 312 | Anti-ATP synthase subunit gamma, rabbit antibodies

AS05 071 | Anti-ATP synthase subunit c, rabbit antibodies

AS12 1853 | Anti-ATP synthase subunit c, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Product citations

Selected references Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Liu et al. (2011). Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance. J Plant Physiol. Nov 7

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