AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1

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AS10 931 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, R. sativus, B.rapa, B.oleracea


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AS10 931

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product information

AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates. Alternative names: At4g20260, endomembrane-associated protein.


KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, At4g20260

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

antibodies to plasma membrane proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Serum contains less than 0.1 % of sodium azide as preservative.

application information
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

24.5 | 36 kDa

Confirmed reactivity Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var. glabra Regel (43 kDa), Brassica oleracea var. italica (41 kDa)
Predicted reactivity Brassicaceae
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1. In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined. Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting. For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band. 

Protocol for preparation of crude membranes 

This protocol will help to prepare crude membrane fractions from very small amount of plant tissue.
(1) Homogenize roots or root tips with the buffer without SDS.
(2) Centrifuge the homogenate in Eppendorf tubes at 2 000 rpm for 2 min to remove cell wall, nuclei, plastids and mitochondria.
(3) Centrifuge the obtained supernatant at 30 000 g for 20 min to separate soluble components. Please use adequate tubes for ultracentrifugation.
(4) Suspend the obtained precipitate (crude membranes) with SDS buffer with DTT or beta-mercaptoethanol.
(5) Heat the sample solution obtained the step (4) at 70 C for 5 min.
(6) Apply the denatured sample to SDS-PAGE.

Courtesy Prof. Msayoshi Maeshima, Nagoya University, Japan
Selected references Ide et al. (2007) Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane. J. Exp. Botany, 58: 1173-1183.
Nagasaki et al. (2008) A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the posphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2. FEBS J. 275: 2267-2282.

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