AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1

KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence UniProt: Q96262, TAIR: At4g20260

24.5 | 36 kDa

Serum contains less than 0.1 % of sodium azide as preservative.

It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1. In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined. Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting. For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band. 

Protocol for preparation of crude membranes 

antibodies to plasma membrane proteins

Plant protein extraction buffer

Secondary antibodies

AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates. Alternative names: At4g20260, endomembrane-associated protein.