SIP2 | Small basic intrinsic protein 2-1

AS09 496 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana SIP2;1 Q9M1K3, AT3G56950

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Liquid

Quantity 100 µl

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

25.9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot using anti-AtSIP2;1 antibodies

crude membrane fraction/lane from Arabidopsis thaliana stems (1,3) and crude membrane fraction from yeast cells expressing SIP2;1 (2,4) were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtSIP2;1 antibodies (AS09 496, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicit anti-SIP2;1 antibodies have been incubated with antibodies - right panel.

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Related products

AS09 495 | antiAtSIP1;1 | aquaporin SIP1-1

collection of antibodies to endomembrane system

Plant protein extraction buffer

Background

SIP protein functions as a water channel to facilitate the transport of water across cell membrane. It is mainly localized to ER. Alternative name: small basic intrinistic protein 2-1

Product citations

Selected references Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.
Ishikawa et al. (2005). Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579:5814-5820.