AMBP | Bikunin (mice)

Name: AMBP | Bikunin (mice)
SKU: AS04 040
Price: 463 EUR
Availability: InStock

AS04 040 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mice

AMBP | Bikunin (mice) in the group Antibodies Human Cell Biology / Other proteins at Agrisera AB (Antibodies for research) (AS04 040)

Product Information

Immunogen

Recombinant mouse bikunin purified from baculovirus infected insect cell cultures

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 2000 (ELISA ), Assay dependent dilution (IHC)

Expected | apparent MW

Free bikunin appears as a smear between 60-80 kDa, because the protein core carries sulfated proteoglycans.

The most common bikunin complexes are called pre-alpha-inhibitor (PaI) and inter-alpha-inhibitor (IaI) and appears as approximately 120 and 180 kDa.

Reactivity

Confirmed reactivity Mouse

Predicted reactivity Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-mouse bikunin antibody

Panel A: Coomasie stained gel. Panel B: 5 ug human (1), rat (2) or mouse (3) bikunin, expressed and purified from recombinant insect cells as described (ref 1), 8 µg of total protein from human (4), rat (5) or mouse (6) serum were mixed in sample buffer, containing 2% SDS and 1% mercaptoethanol, and denatured at 100°C for 2 min, and then separated on pre-cast 4-20 % gels bought from Bio-Rad (Mini-PROTEAN Tris-Glycine, Stain-Free, 4-20%) and blotted PVDF using semi-dry transfer. The membranes were blocked with 1% gelatin buffer 20 min at room temperature (RT) with agitation, and then incubated in the primary antibody at a dilution of 1: 2000 in 0.5% gelatin and 0.25% Tween-20 overnight at 4°C with agitation. The antibody solution was decanted and the membrane was rinsed 4x7 min with in 0.5% gelatin and 0.25% Tween-20, and incubated in secondary antibody (goat anti-rabbit IgG-Alexa 647, from Molecular Probes Inc) diluted to 10 ug/ml in 0.5% gelatin and 0.25% Tween-20 for 1h at RT with agitation. The blot was washed as above and developed using a ChemiDoc™ MP System (Bio-Rad) with the Alexa 650-settings.

Free bikunin appears as a smear between 60-80 kDa, because the protein core carries sulfated proteoglycans.

The most common bikunin complexes are called pre-alpha-inhibitor (PaI) and inter-alpha-inhibitor (IaI) and appears as approximately 120 and 180 kDa, respectively. Zho et al. (2004). Inter-alpha-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex. J Biol Chem. 2004 Sep 10;279(37):38079-82.

Reference 1. Falkenberg et al. (2001). Expression of a functional proteinase inhibitor capable of accepting xylose: bikunin. Arch Biochem Biophys 387: 99-106.

Additional information

Additional information This antibody is also reacting with the AMBP precursor

Background

Bikunin is a Kunitz-type protease inhibitor synthesized in the liver. The AMBP gene resides in the lipocalin cluster and encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Bikunin exhibits anti-inflammatory activity in protection against cancer and inflammation.

Product citations

Selected references Sánchez et al. (2002). Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis. Mech Dev. 2002 Sep;117(1-2):293-8.

immunogen:	Recombinant mouse bikunin purified from baculovirus infected insect cell cultures
Reconstitution:	For reconstitution add 200 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	200 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 2000 (ELISA ), Assay dependent dilution (IHC)
calculated \| apparent molecular mass [kDa]:	Free bikunin appears as a smear between 60-80 kDa, because the protein core carries sulfated proteoglycans. The most common bikunin complexes are called pre-alpha-inhibitor (PaI) and inter-alpha-inhibitor (IaI) and appears as approximately 120 and 180 kDa.
Confirmed reactivity:	Mouse
predicted reactivity:	Rat
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Panel A: Coomasie stained gel. Panel B: 5 ug human (1), rat (2) or mouse (3) bikunin, expressed and purified from recombinant insect cells as described (ref 1), 8 µg of total protein from human (4), rat (5) or mouse (6) serum were mixed in sample buffer, containing 2% SDS and 1% mercaptoethanol, and denatured at 100°C for 2 min, and then separated on pre-cast 4-20 % gels bought from Bio-Rad (Mini-PROTEAN Tris-Glycine, Stain-Free, 4-20%) and blotted PVDF using semi-dry transfer. The membranes were blocked with 1% gelatin buffer 20 min at room temperature (RT) with agitation, and then incubated in the primary antibody at a dilution of 1: 2000 in 0.5% gelatin and 0.25% Tween-20 overnight at 4°C with agitation. The antibody solution was decanted and the membrane was rinsed 4x7 min with in 0.5% gelatin and 0.25% Tween-20, and incubated in secondary antibody (goat anti-rabbit IgG-Alexa 647, from Molecular Probes Inc) diluted to 10 ug/ml in 0.5% gelatin and 0.25% Tween-20 for 1h at RT with agitation. The blot was washed as above and developed using a ChemiDoc™ MP System (Bio-Rad) with the Alexa 650-settings. Free bikunin appears as a smear between 60-80 kDa, because the protein core carries sulfated proteoglycans. The most common bikunin complexes are called pre-alpha-inhibitor (PaI) and inter-alpha-inhibitor (IaI) and appears as approximately 120 and 180 kDa, respectively. Zho et al. (2004). Inter-alpha-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex. J Biol Chem. 2004 Sep 10;279(37):38079-82. Reference 1. Falkenberg et al. (2001). Expression of a functional proteinase inhibitor capable of accepting xylose: bikunin. Arch Biochem Biophys 387: 99-106.
additional information:	This antibody is also reacting with the AMBP precursor
background:	Bikunin is a Kunitz-type protease inhibitor synthesized in the liver. The AMBP gene resides in the lipocalin cluster and encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Bikunin exhibits anti-inflammatory activity in protection against cancer and inflammation.
All references:	Sánchez et al. (2002). Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis. Mech Dev. 2002 Sep;117(1-2):293-8.