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Biotin carboxyl carrier subunit of chloroplast ACCase

AS15 2881  | Clonality: Polyclonal  |  Host: Guinea pig  |  Reactivity: Pisum sativum, Arabidobsis, Hordeum vulgare, Solanum lycopersicum

Biotin carboxyl carrier subunit of chloroplast ACCase in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS15 2881)

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Product Information

Immunogen

Purified native protein from Pisum sativum

Host Guinea pig
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

29.6 kDa

Reactivity

Confirmed reactivity Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
Predicted reactivity

Hordeum vulgare, Nicotiana tabacum


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
20 µg of a total cell extract leaves of Nicotiana tabacum (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) were separated on 12 % SDS-PAGE  and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

Additional information

Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.

Related products

Related products

AS15 2880 | Anti-ACCase subunit beta | acetyl-coenzyme A, carboxylase (subunit beta) rabbit antibodies

Secondary antibodies

Background

Background Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.

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