Biotin carboxyl carrier subunit of chloroplast ACCase
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Purified native protein from Pisum sativum
For reconstitution add 100 µl of sterile water.
Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Hordeum vulgare, Nicotiana tabacum
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20 µg of a total cell extract leaves of Nicotiana tabacum (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.
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