Biotin carboxyl carrier subunit of chloroplast ACCase

AS15 2881 | Clonality: Polyclonal | Host: Guinea pig | Reactivity: Pisum sativum, Arabidobsis, Hordeum vulgare, Solanum lycopersicum

Biotin carboxyl carrier subunit of chloroplast ACCase

Product Information

Immunogen

Purified native protein from Pisum sativum

Host Guinea pig

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW 29.6 kDa

Reactivity

Confirmed reactivity Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum

Predicted reactivity

Hordeum vulgare, Nicotiana tabacum

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

20 µg of a total cell extract leaves of Nicotiana tabacum (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

Additional information

Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.

Background

Background Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.

Product citations

background:	Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
additional information (application):	Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.
calculated \| apparent molecular mass [kDa]:	29.6 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Guinea pig
immunogen:	Purified native protein from Pisum sativum
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 2000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
predicted reactivity:	Hordeum vulgare, Nicotiana tabacum Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	20 µg of a total cell extract leaves of Nicotiana tabacum (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.