BrdU | Bromodeoxyuridine

AS16 3995 | Clonality: Polyclonal | Host: Sheep | Reactivity: Bromodeoxyuridine

Product Information

Immunogen Bromodeoxyuridine, conjugated to Helix Pomatia Haemocyanin,

Host Sheep

Clonality Polyclonal

Purity Total IgG. Purified using ammonium sulfate precipitation.

Format Liquid

Quantity 250 µg

Storage Store at -20°C.

Tested applications Immunohistochemistry(IHC), Immunoprecipitation (IP), Western blot(WB)

Recommended dilution 25-100 µg/ml (IHC), 25-100 µg/ml (IP), 25 -100 µg/ml (WB)

Reactivity

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Provided in PBS, contains 0.08% sodium azide.

The product has been tested in IP against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU). This antibody shows a 8-fold higher reactivity with 5-MeC versus BrdU, at a concentration of 25 ug/ml.

Please note: Denature the DNA sample first to make the bases accessible to the BrdU antibody.

Background

Bromodeoxyuridine is a synthetic nucleoside that is an analog of thymidine which can be integrated into the DNA of proliferating cells as a marker to detect proliferating cells in living tissues.

Product citations

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