Bromelain (affinity purified)
AS09 554 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus

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Product Information
Immunogen
native bromelain isolated and purified from Ananas comosus
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
1 mg
Reconstitution
For reconstitution add 0.5 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Reactivity
Confirmed reactivity
Ananas comosus
Predicted reactivity
Ananas comosus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Background
Background
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product citations
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