BSA | Bovine serum albumin (affinity purified, rabbit antibody)

Product no: AS99 002

AS99 002  |  Clonality: polyclonad  |  Host: Rabbit  |  Reactivity: Bovine, Rat

299
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    purified BSA, whole molecule P02769

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 100 g
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 5 000 (ELISA), 1 : 5 000 (WB)
    Expected | apparent MW:

    67 kDa

  • Reactivity
  • Confirmed reactivity: Bovine, Rat
    Predicted reactivity: Goat, Sheep
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    Indirect ELISA: Anti-BSA antibodies (0.1 mg/ml) at dilution of 1:5 000 was found to generate absorbance 1.0 at 650 nm after 10 minutes of incubation with DAKO® TMB One-Step Substrate System at room temperature.

  • Additional Information
  • Additional information: Low endotoxin level as determined by Lonza LAL kit and compare to other products
    Additional information (application): Antibody is recognising endogenous rat serum albumin
  • Background
  • Background:

    Albumin is the most abundant protein in the circulatory system. In mammals albumin is synthesized initially as preproalbumin by the liver. After removal of the signal peptide, the resultant proalbumin is further processed by removal of the six-residue propeptide from the new N-terminus. The albumin released into circulation possesses a half-life of 19 days.

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