BSA | Bovine serum albumin (affinity purified, rabbit antibody)

AS99 002 | Clonality: polyclonad | Host: Rabbit | Reactivity: Bovine, Rat

Product Information

Immunogen

purified BSA, whole molecule P02769

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 5 000 (ELISA), 1 : 5 000 (WB)

Expected | apparent MW

67 kDa

Reactivity

Confirmed reactivity Bovine, Rat

Predicted reactivity Goat, Sheep

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

Indirect ELISA: Anti-BSA antibodies (0.1 mg/ml) at dilution of 1:5 000 was found to generate absorbance 1.0 at 650 nm after 10 minutes of incubation with DAKO^® TMB One-Step Substrate System at room temperature.

Additional information

Additional information Low endotoxin level as determined by Lonza LAL kit and compare to other products

Antibody is recognising endogenous rat serum albumin

Background

Albumin is the most abundant protein in the circulatory system. In mammals albumin is synthesized initially as preproalbumin by the liver. After removal of the signal peptide, the resultant proalbumin is further processed by removal of the six-residue propeptide from the new N-terminus. The albumin released into circulation possesses a half-life of 19 days.

Product citations

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Confirmed reactivity:	Bovine, Rat
predicted reactivity:	Goat, Sheep
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Low endotoxin level as determined by Lonza LAL kit and compare to other products
additional information (application):	Antibody is recognising endogenous rat serum albumin
background:	Albumin is the most abundant protein in the circulatory system. In mammals albumin is synthesized initially as preproalbumin by the liver. After removal of the signal peptide, the resultant proalbumin is further processed by removal of the six-residue propeptide from the new N-terminus. The albumin released into circulation possesses a half-life of 19 days.
calculated \| apparent molecular mass [kDa]:	67 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	purified BSA, whole molecule P02769
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	100 µg
recommended dilution:	1 : 5 000 (ELISA), 1 : 5 000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
Picture (footer):	application example Indirect ELISA: Anti-BSA antibodies (0.1 mg/ml) at dilution of 1:5 000 was found to generate absorbance 1.0 at 650 nm after 10 minutes of incubation with DAKO^® TMB One-Step Substrate System at room temperature.