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c-myc (monoclonal, Clone 9E10)

AS20 4454 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins

c-myc (monoclonal, Clone 9E10) in the group Tag Antibodies / Myc at Agrisera AB (Antibodies for research) (AS20 4454)

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Product Information

Immunogen Synthetic peptide EQKLISEEDL coupled to a carrier
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein A/G purified
Format Liquid in PBS with 50 % glycerol, pH 7.5 and 3 mM sodium azide
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescense (IF), Western Blot (WB),
Recommended dilution 1-5 µg/ml (ELISA), (WB)

Reactivity

Confirmed reactivity  Myc epitope tag in recombinant proteins

Application examples

Additional information

Related products

Background

Background The anti-c-myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc.  Clone 9E10 anti-c-myc can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells.

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