c-myc (monoclonal, Clone 9E10)
AS20 4454 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins
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Product Information
Immunogen
Synthetic peptide EQKLISEEDL coupled to a carrier
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Protein G purified
Format
Liquid in PBS with 50 % glycerol, pH 7.5 and 3 mM sodium azide
Quantity
100 µg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescense (IF), Western Blot (WB),
Recommended dilution
1-5 µg/ml (ELISA), (WB)
Reactivity
Confirmed reactivity
Myc epitope tag in recombinant proteins
Application examples
Additional information
Background
Background
The anti-c-myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc. Clone 9E10 anti-c-myc can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells.
Product citations
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