c-myc (monoclonal, Clone 9E10)
AS20 4454 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins
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Immunogen Synthetic peptide EQKLISEEDL coupled to a carrier
Purity Protein G purified
Format Liquid in PBS with 50 % glycerol, pH 7.5 and 3 mM sodium azide
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescense (IF), Western Blot (WB),
Recommended dilution 1-5 µg/ml (ELISA), (WB)
Confirmed reactivity Myc epitope tag in recombinant proteins
Background The anti-c-myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc. Clone 9E10 anti-c-myc can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells.
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