showForm Anti-Ca2+-ATPase | calmodulin-stimulated calcium-ATPase antibodies

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Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase

AS09 486 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase in the group Antibodies for Plant/Algal  / Membrane Transport System / Plasma membrane at Agrisera AB (Antibodies for research) (AS09 486)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase P93067

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 µl
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

111 | 110 kDa (Raphanus sativus)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea, Raphanus sativus
Predicted reactivity Oryza sativa, Physcomitrella patens, Populus balsamifera, Triticum aestivum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using Ca2+ ATPase antibodies

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase  antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

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Background

Background

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type).

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