Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase

AS09 486 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase P93067

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Liquid

Quantity 100 µl

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

111 | 110 kDa (Raphanus sativus)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Predicted reactivity Oryza sativa, Physcomitrella patens, Populus balsamifera, Triticum aestivum, Vitis vinifera

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot using Ca2+ ATPase antibodies

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.