Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase

AS09 486 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase UniProt:P93067

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

111 | 110 kDa (Raphanus sativus)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Predicted reactivity Oryza sativa, Physcomitrella patens, Populus balsamifera, Triticum aestivum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

western blot using Ca2+ ATPase antibodies

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Related products

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Background

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type).

Product citations

Selected references Siddiqui et al. (2020). Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress. Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882