Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase UniProt:P93067

111 | 110 kDa (Raphanus sativus)

Application example

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase  antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type).