Capsaicin receptor

AS13 2701 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Mouse, Rat

Product Information

Immunogen A synthetic peptide of human capsaicin receptor protein conjugated to KLH has been used as the immunogen,

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications Immunohistochemistry (IHC)

Recommended dilution 1 : 1000-1 : 2000 (IHC)

Reactivity

Confirmed reactivity Human, Mouse, Rat

Application examples

Application examples Application example

Immunohistochemical staining of rat spinal cord using rabbit antibody to human capsaicin receptor, catalog number AS13 2701.

immunofluorescence of neurofilaments with capsaicin antibodies

immunofluorescence of neurofilaments with capsaicin antibodies

Immunohistochemistry showing capsaicin receptor (TRPV1, red) and neurofilament-heavy chain (NFL, green) reactivity in nociceptive sensory neurons derived from hESC. Fixation: 4% PFA 10 min. on ice. Blocking: 10% FCS/PBST for 1h at RT Primary: 1:100 in blocking solution for 1h at RT Secondary: 1:500 in blocking solution for 1h at RT, secondary antibodies: donkey anti-mouse 488 and donkey anti-rabbit 568.

Courtesy of Dr. Iwan Jones, Umeå University, Sweden

Additional information

Background

Capsaicin receptor is predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells. Synonyms: The transient receptor potential cation channel subfamily V member 1 (TrpV1) and the vanilloid receptor 1

Product citations

Selected references Jones et al. (2018). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.

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Picture (footer):	Application example Immunohistochemical staining of rat spinal cord using rabbit antibody to human capsaicin receptor, catalog number AS13 2701. Immunohistochemistry showing capsaicin receptor (TRPV1, red) and neurofilament-heavy chain (NFL, green) reactivity in nociceptive sensory neurons derived from hESC. Fixation: 4% PFA 10 min. on ice. Blocking: 10% FCS/PBST for 1h at RT Primary: 1:100 in blocking solution for 1h at RT Secondary: 1:500 in blocking solution for 1h at RT, secondary antibodies: donkey anti-mouse 488 and donkey anti-rabbit 568. Courtesy of Dr. Iwan Jones, Umeå University, Sweden
Confirmed reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	A synthetic peptide of human capsaicin receptor protein conjugated to KLH has been used as the immunogen,
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 1000-1 : 2000 (IHC)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
tested applications:	Immunohistochemistry (IHC)
background:	Capsaicin receptor is predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells. Synonyms: The transient receptor potential cation channel subfamily V member 1 (TrpV1) and the vanilloid receptor 1
All references:	Jones et al. (2018). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.