IKP

CD62P | P-selectin, labelled with fluorescein

Product no: IMS09-143-338

IMS09-143-338    Clonality: Polyclonal    Host: Hen    Reactivity: Human

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  • Product Info
  • Immunogen:

    recombinant human P-selectin UniProt:P16109

    Host: Chicken
    Clonality: Polyclonal
    Purity: Total IgY in 0.9% NaCl, 0.1% sodium azide.
    Format: Liquid
    Quantity: 100 l (0,7 mg/ml)
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Flow cytometry (Flow cyt), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    91 kDa

  • Reactivity
  • Confirmed reactivity: Human, Porcine
    Predicted reactivity: Horse
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with fluorescein
    Additional information (application):

    The antibodies have been tested in flow cytometry to provide reproducible results; antibodies will react with activated human and porcine platelets according to the method Larsson A et al. Platelets. 2002 May;13(3):153-7 Studies of fibrinogen binding to porcine platelets by flow cytometry: a method for studies of porcine platelet activation.

    Flow cytometry: Suitable for detection of platelet activation by flow cytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704, Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated by centrifugation at room temperature. 5 mL platelet-rich plasma was added to polystyrene tubes containing 100 mL HEPES-buffer (137 mmol/L NaCl, 2.7 mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albumin and 20 mmol/L HEPES, pH 7.4) and 10 mL FITC labelled chicken antibody. The samples were incubated for 10 minutes at room temperature and were then diluted and fixed with 1000 mL ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/L NaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing steps were used. The samples were analyzed utilising an Epics Profile XL-MCL cytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 platelets was carried out with the XL software (Coulter Electronics)

  • Background
  • Background:

    Platelets or thrombocytes are the cell fragments circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. Platelets are produced in the bone marrow; the progenitor cell for platelets is the megakaryocyte. It is about twelve times larger than an erythrocyte, possesses a lobed nucleus and sheds platelets into the circulation. Alternative protein names include: CD62P; GMP140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; LECAM3; Leukocyte endothelial cell adhesion molecule 3; PADGEM; Platelet activation dependent granule external membrane protein; PSEL; SELP

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Accessories

AS10 1489 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken IgG/Y (H&L)

10 % until end of 2023. Use discount code: Conj10

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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

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