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CD62P | P-selectin, labelled with fluorescein

IMS09-143-338    Clonality: Polyclonal    Host: Hen    Reactivity: Human

CD62P | P-selectin, labelled with fluorescein in the group Antibodies for Human/Animal  / Human Proteins / Cardiovascular at Agrisera AB (Antibodies for research) (IMS09-143-338)

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Product Information

Immunogen

recombinant human P-selectin UniProt:P16109

Host Chicken
Clonality Polyclonal
Purity Total IgY fraction
Format Liquid in 0.9 % NaCl, 0.1 % sodium azide
Quantity 100 µl (0.7 mg/ml)
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Flow cytometry (Flow cyt), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

91 kDa

Reactivity

Confirmed reactivity Human, Porcine
Predicted reactivity Horse
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with fluorescein.

The antibodies have been tested in flow cytometry to provide reproducible results; antibodies will react with activated human and porcine platelets according to the method Larsson A et al. Platelets. 2002 May;13(3):153-7 Studies of fibrinogen binding to porcine platelets by flow cytometry: a method for studies of porcine platelet activation.

Flow cytometry: Suitable for detection of platelet activation by flow cytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704, Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated by centrifugation at room temperature. 5 mL platelet-rich plasma was added to polystyrene tubes containing 100 mL HEPES-buffer (137 mmol/L NaCl, 2.7 mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albumin and 20 mmol/L HEPES, pH 7.4) and 10 mL FITC labelled chicken antibody. The samples were incubated for 10 minutes at room temperature and were then diluted and fixed with 1000 mL ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/L NaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing steps were used. The samples were analyzed utilising an Epics Profile XL-MCL cytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 platelets was carried out with the XL software (Coulter Electronics)

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Background

Background

Platelets or thrombocytes are the cell fragments circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. Platelets are produced in the bone marrow; the progenitor cell for platelets is the megakaryocyte. It is about twelve times larger than an erythrocyte, possesses a lobed nucleus and sheds platelets into the circulation. Alternative protein names include: CD62P; GMP140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; LECAM3; Leukocyte endothelial cell adhesion molecule 3; PADGEM; Platelet activation dependent granule external membrane protein; PSEL; SELP

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