Chlorpyrifos (polyclonal antibody)
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The titer in the indirect ELISA using OVA-conjugate was 500, in the indirect ELISA it was 100 000.
The antibody is highly specific for chlorpyriphos showing no cross-reactivity with related and unrelated compounds. In the direct ELISA the detection limit was 1-4 nM (0.3-1.3 µg/L).
Affinity and avidity were not determined. Specific antibody concentration is not determined as it is provided as a total immunoglobulin pool (purified by caprilic acid).
Caprilic acid purified immunoglobulins can be used for most procedures like: coating plates for antigen capture assays and preparation of immunoaffinity columns. However, such preparations are not suitable for: conjugation with radioisotopes, enzymes, and biotin where contaminating proteins will reduce the specific activity..
Chlorpyrifos is an insecticide belonging to the group of organophosphothioates. It is used in the culture of vegetables and in citrus orchards. Chlorpyrifos is an acetylcholinesterase inhibitor and as such having a highly toxic effect on the nervous system. There is evidence that chlorpyrifos has an effect on the foetus, which may lead to behavioural disorders.Chlorpyrifos, CAS no.: 2921-88-2, Solubility: Methanol, Iso-octanol, Benzene.
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