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Chlorpyrifos (polyclonal antibody)

AS13 2736  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlorpyrifos
Chlorpyrifos (polyclonal antibody) in the group Antibodies for Plant/Algal  / Toxins / Insecticides at Agrisera AB (Antibodies for research) (AS13 2736)

DATA SHEET IN PDF

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401
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen
BSA-conjugated Chlorpyrifos, Target: Chlorpyrifos, CAS no.: 2921-88-2 purchased from SIGMA
Host Rabbit
Clonality Polyclonal
Purity IgG
Format Liquid
Quantity 1 ml
Storage Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.
Tested applications ELISA (ELISA), Immuno-affinity-chromatography (IAC)
Recommended dilution 1 : 500 (ELISA)

Reactivity

Application examples

Additional information

Additional information
AS13 2733 | Alkylphenols (nonylphenol; octylphenol), rabbit antibodies
ELISA titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested in this technique. Plates are coated with 400 ng/ml ovalbumin conjugated-chlorpyrifos. Detection antibody is: anti-rabbit IgG, HRP-conjugated antibody in dilution 1: 8000.

chlorpyrifos cross reactivity table The titer in the indirect ELISA using OVA-conjugate was 500, in the indirect ELISA it was 100 000.

The antibody is highly specific for chlorpyriphos showing no cross-reactivity with related and unrelated compounds. In the direct ELISA the detection limit was 1-4 nM (0.3-1.3 µg/L).

Affinity and avidity were not determined. Specific antibody concentration is not determined as it is provided as a total immunoglobulin pool (purified by caprilic acid). 

Caprilic acid purified immunoglobulins can be used for most procedures like: coating plates for antigen capture assays and preparation of immunoaffinity columns. However, such preparations are not suitable for: conjugation with radioisotopes, enzymes, and biotin where contaminating proteins will reduce the specific activity..

Related products

Related products AS19 4363 | Chlorpyrifos (antigen), trial sample

Secondary antibodies

Background

Background

Chlorpyrifos is an insecticide belonging to the group of organophosphothioates. It is used in the culture of vegetables and in citrus orchards. Chlorpyrifos is an acetylcholinesterase inhibitor and as such having a highly toxic effect on the nervous system. There is evidence that chlorpyrifos has an effect on the foetus, which may lead to behavioural disorders.

Product citations

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