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pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1

AS15 3077  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, S. lycopersicum, S. oleracea,

pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1 in the group Antibodies for Plant/Algal  / Photosynthesis  / Proteases at Agrisera AB (Antibodies for research) (AS15 3077)

DATA SHEET IN PDF

Qty: 
380
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Product Information

Immunogen Full length, recombinant pClpP protein from Arabidopsis thaliana, UniPro: P56772, TAIR: ATCG00670
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

22 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum
Predicted reactivity Aethionema grandiflorum, Arabis hirsuta, Barbarea verna, Brassica napus, Capsella bursa-pastoris, Cardamine impatiens, Carica papaya, Coffea arabica, Crucihimalaya wallichii, Daucus carota, Draba nemorosa, Ehretia acuminata, Ellisia nyctelea, Forsythia europaea, Hesperelaea palmeri, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Nicotiana tabacum, Olea europaea subsp. maroccana, Olimarabidopsis pumila , Pachycladon cheesemanii, Raphanus sativus, Scutellaria baicalensis
Species of your interest not listed? Contact us
Not reactive in

Hordeum vulgare

Application examples

Application examples application example

western blot using anti-ClpP antibodies
20 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-ClpP antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

Additional information

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Background

Background pClpP (Chloroplastic ATP-dependent Clp protease proteolytic subunit 1) has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.

Product citations

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