pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1

Name: pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1
Brand: IKP
SKU: AS15 3077
Price: 402 EUR
Availability: InStock

AS15 3077 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, S. lycopersicum, S. oleracea,

pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1 in the group Antibodies Plant/Algal / Photosynthesis / Proteases at Agrisera AB (Antibodies for research) (AS15 3077)

pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1

Product Information

Immunogen Full length, recombinant pClpP protein from Arabidopsis thaliana, UniPro: P56772, TAIR: ATCG00670

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5 000 (WB)

Expected | apparent MW

22 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum

Predicted reactivity Aethionema grandiflorum, Arabis hirsuta, Barbarea verna, Brassica napus, Capsella bursa-pastoris, Cardamine impatiens, Carica papaya, Coffea arabica, Crucihimalaya wallichii, Daucus carota, Draba nemorosa, Ehretia acuminata, Ellisia nyctelea, Forsythia europaea, Hesperelaea palmeri, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Nicotiana tabacum, Olea europaea subsp. maroccana, Olimarabidopsis pumila , Pachycladon cheesemanii, Raphanus sativus, Scutellaria baicalensis
Species of your interest not listed? Contact us

Not reactive in

Hordeum vulgare

Application examples

Application examples application example

western blot using anti-ClpP antibodies

20 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-ClpP antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

Additional information

Background

Background pClpP (Chloroplastic ATP-dependent Clp protease proteolytic subunit 1) has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.

Product citations

immunogen:	Full length, recombinant pClpP protein from Arabidopsis thaliana, UniPro: P56772, TAIR: ATCG00670
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 5 000 (WB)
calculated \| apparent molecular mass [kDa]:	22 kDa
Confirmed reactivity:	Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum
predicted reactivity:	Aethionema grandiflorum, Arabis hirsuta, Barbarea verna, Brassica napus, Capsella bursa-pastoris, Cardamine impatiens, Carica papaya, Coffea arabica, Crucihimalaya wallichii, Daucus carota, Draba nemorosa, Ehretia acuminata, Ellisia nyctelea, Forsythia europaea, Hesperelaea palmeri, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Nicotiana tabacum, Olea europaea subsp. maroccana, Olimarabidopsis pumila , Pachycladon cheesemanii, Raphanus sativus, Scutellaria baicalensis Species of your interest not listed? Contact us
not reactive in:	Hordeum vulgare
Picture (footer):	application example 20 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-ClpP antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.
background:	pClpP (Chloroplastic ATP-dependent Clp protease proteolytic subunit 1) has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.

Related products: pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

ClpB-P | ClpB3

AS09 459 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana

382 €

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

HSP101 | ClpB heat shock protein, N-terminal (rabbit antibody)

AS07 253 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A. tequilana var. Weber,...

302 €