pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1
AS15 3077 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, S. lycopersicum, S. oleracea,
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20 µg of total protein from leaf extract from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-ClpP antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.
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