IKP

CO4 | Complement component C4, Biotin conjugated

Product no: IMS06-032-304

IMS06-032-304  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Human 

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    full length native protein UniProt:P0C0L4 purified from human plasma

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide., pH 7.2.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: Use at an assay dependent dilution (WB)
    Expected | apparent MW:

    193 kDa

  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Human
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Affinity purified on human C4 agaroseconcentration: 1 mg/ml
    Additional information (application): Prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum or human C4, Only one precipitin arc in immunoelectrophoresis against normal human serum or human C4, Specific against human C4 alpha and beta chains in electroblotting when tested against normal human serum
  • Background
  • Background:

    Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

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