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CO4 | Complement component C4, Biotin conjugated

IMS06-032-304  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Human 

CO4 | Complement component C4, Biotin conjugated in the group Antibodies for Human/Animal  / Human Proteins / Immunology at Agrisera AB (Antibodies for research) (IMS06-032-304)

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Product Information

Immunogen

full length native protein UniProt:P0C0L4 purified from human plasma

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Quantity 100 µl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution Use at an assay dependent dilution (WB)
Expected | apparent MW

193 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Human
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Affinity purified on human C4 agaroseconcentration: 1 mg/ml

Prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum or human C4. Only one precipitin arc in immunoelectrophoresis against normal human serum or human C4. Specific against human C4 alpha and beta chains in electroblotting when tested against normal human serum.

Related products

Related products

IMS02-032-303 | Anti-Complement component C4, chicken antibodies

IMS01-031-305 | Anti-Complement component C4c, chicken antibodies

IMS01-066-309 | Anti-Complement factor H, chicken antibodies

Background

Background

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Product citations

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