CO4 | Complement component C4

IMS02-032-303 | Clonality: Polyclonal | Host: Hen | Reactivity: Human

Product Information

Immunogen

full length native protein P0C0L4 purified from human plasma

Host Chicken

Clonality Polyclonal

Purity Affinity purified IgY

Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2

Quantity 100 µl (1 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Affinity purified IgY

Recommended dilution 1 : 2000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

193 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Human

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Affinity purified IgY on human C4 agarose

Only one precipitin arc in immunodiffusion against normal human serum or human complement component C4. Only one precipitin arc in immunoelectrophoresis against normal human serum or human complement component C4.

ELISA has been performed on C4 deposition using human and mouse serum.

Related products

IMS06-032-304 | anti-Complement component C4, biotinylated

IMS01-031-305 | anti-Complement component C4c

IMS01-066-309 | anti-Complement factor H

Secondary antibodies

Background

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.