CO4c | Complement component C4c

IMS01-031-305 | Clonality: Polyclonal | Host: Hen | Reactivity: Human

Product Information

Immunogen

full lenth native CO4c | Complement component C4c protein purified from human plasma

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.

Format Liquid

Quantity 100 µl (25,9 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Total, purified IgY

Recommended dilution Use at an assay dependent dilution (WB)

Expected | apparent MW

35 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Human

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation, procedure followed by ammonium sulphate precipitation

Only one precipitin arcs in immunodiffusion against normal human serum or human C4. Reacts with α and β chain in electroblotting.

The names C4 and C4c for this target protein are frequently used together and almost interchangeably. The difference between the two is a single cleavage and the difference will not be able to be discerned in a polyclonal antibody.

Background

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Product citations

Selected references Bidula et al. (2013). The role of ficolin-A and lectin complement pathway in the innate defence against pathogenic Aspergillus species. Infect. Immun. March 11.

Confirmed reactivity:	Human
predicted reactivity:	Human
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
additional information:	The IgY fraction is isolated by a two-step PEG precipitation, procedure followed by ammonium sulphate precipitation
additional information (application):	Only one precipitin arcs in immunodiffusion against normal human serum or human C4. Reacts with α and β chain in electroblotting. The names C4 and C4c for this target protein are frequently used together and almost interchangeably. The difference between the two is a single cleavage and the difference will not be able to be discerned in a polyclonal antibody.
All references:	Bidula et al. (2013). The role of ficolin-A and lectin complement pathway in the innate defence against pathogenic Aspergillus species. Infect. Immun. March 11.
calculated \| apparent molecular mass [kDa]:	35 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Chicken
immunogen:	full lenth native CO4c \| Complement component C4c protein purified from human plasma
Purity:	Purified, total IgY (chicken egg yolk immunoglobulin) in 0.9% NaCl, 0.02% sodium azide.
Quantity:	100 µl (25,9 mg/ml)
recommended dilution:	Use at an assay dependent dilution (WB)
storage:	Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Total, purified IgY

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