DISCONTINUED COXII | Cytochrome oxidase subunit II (200 µg)

Name: DISCONTINUED COXII | Cytochrome oxidase subunit II (200 µg)
SKU: AS04 053A-200
Price: 767 EUR
Availability: OutOfStock

AS04 053A-200 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Eudicots, monocots, Physcomitrella patens | cellular [compartment marker] of mitochondrial inner membrane

Replaced by AS04 053A

DISCONTINUED COXII | Cytochrome oxidase subunit II (200 µg) in the group Antibodies Plant/Algal / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS04 053A-200)

DISCONTINUED COXII | Cytochrome oxidase subunit II (200 µg)

Product Information

Immunogen

KLH-conjugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativaP04373 and Physcomitrella patens Q1XGA9

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 130 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica

Predicted reactivity Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.

western blot detection using anti-COXII antibody

Additional information

Additional information Cellular [compartment marker] of mitochondrial inner membrane

Antibody detects COXII protein in total leaf extracts and isolated mitochondria.

Background

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Product citations

Selected references Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.
Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.

immunogen:

Reconstitution:

For reconstitution add 130 µl of sterile water

Host:

Rabbit

Clonality:

Polyclonal

Purity:

Immunogen affinity purified serum in PBS pH 7.4.

Format:

Lyophilized

Quantity:

200 µg

storage:

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

tested applications:

Western blot (WB)

recommended dilution:

1 : 1000 (WB)

calculated | apparent molecular mass [kDa]:

29.4 | 30 kDa (for Arabidopsis thaliana)

Confirmed reactivity:

Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica

predicted reactivity:

Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Zea mays
Species of your interest not listed? Contact us

not reactive in:

No confirmed exceptions from predicted reactivity are currently known

Picture (footer):

application example

additional information:

Cellular [compartment marker] of mitochondrial inner membrane

Antibody detects COXII protein in total leaf extracts and isolated mitochondria.

background:

All references:

Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.
Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.

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