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COXII | Cytochrome oxidase subunit II (200 ĩg)

AS04 053A-200  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: eudicots, monocots, Physcomitrella patens  |  cellular [compartment marker] of mitochondrial inner membrane

COXII | Cytochrome oxidase subunit II (200 ĩg) in the group Plant/Algal Antibodies / Compartment Markers / Mitochondria at Agrisera AB (Antibodies for research) (AS04 053A-200)

PRODUCT INFORMATION IN PDF

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product information
Background

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Immunogen

KLH-conjugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including  Oryza sativaP04373 and Physcomitrella patens Q1XGA9

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 130 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS04 052 anti-COXII hen antibody

AS04 151 ant-COXIIb rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information Cellular [compartment marker] of mitochondrial inner membrane
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica
Predicted reactivity Glycne max, Oryza sativa, Physcomitrella patens, Pisum sativum, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody detects COXII protein in total leaf extracts and isolated mitochondria.
Selected references Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.

application example

 

 

1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.

 

western blot detection using anti-COXII antibody
 

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