COXII | Cytochrome oxidase subunit II (200 ĩg)
AS04 053A-200 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Eudicots, monocots, Physcomitrella patens | cellular [compartment marker] of mitochondrial inner membrane
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KLH-conjugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativaP04373 and Physcomitrella patens Q1XGA9
29.4 | 30 kDa (for Arabidopsis thaliana)
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1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.
Antibody detects COXII protein in total leaf extracts and isolated mitochondria.
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.