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DCL1 | Dicer-like protein 1

AS19 4307  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

DCL1 | Dicer-like protein 1 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS19 4307)

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated synthetic peptides derived from Arabidopsis thaliana DCL1 sequence, Q9SP32 , At1g01040
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µg, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin Immunoprecipiation (ChIP)
Recommended dilution 10 µg (ChIP)
Expected | apparent MW 213 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples ChIP using anti-DCL1 antibodies



Chromatin immunoprecipitation was performed using 4 g of crosslinked (1% formaldehyde) Arabidopsis thaliana tissue as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bioruptor Pico for 15 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragments. Chromatin was immunoprecipitated with a DCL1 antibody (10µg/IP) and Dynabeads®Protein G (Life Technologies). Libraries were prepared using the MicroPlex Library Preparation Kit (Diagenode) and sequenced on the NextSeq platform.

Courtesy Dr. Jakub Dolata, Laboratory of Gene Expression, UAM, Poznań, Poland

Additional information

Related products

Related products AS15 3100 | anti-DCL2 | Dicer-like protein 2, rabbit antibodies
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| anti-DCL3 | Dicer-like protein 3, rabbit antibodies

Background

Background DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs).
Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.

Product citations

Selected references To be added when available, antibody released in July 2020.

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