DCL1 | Dicer-like protein 1
AS19 4307 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptides derived from Arabidopsis thaliana DCL1 sequence, Q9SP32 , At1g01040
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µg, of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin Immunoprecipiation (ChIP)
Recommended dilution
10 µg (ChIP)
Expected | apparent MW
213 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Chromatin immunoprecipitation was performed using 4 g of crosslinked (1% formaldehyde) Arabidopsis thaliana tissue as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bioruptor Pico for 15 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragments. Chromatin was immunoprecipitated with a DCL1 antibody (10µg/IP) and Dynabeads®Protein G (Life Technologies). Libraries were prepared using the MicroPlex Library Preparation Kit (Diagenode) and sequenced on the NextSeq platform.
Courtesy Dr. Jakub Dolata, Laboratory of Gene Expression, UAM, Poznań, Poland
Chromatin immunoprecipitation was performed using 4 g of crosslinked (1% formaldehyde) Arabidopsis thaliana tissue as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bioruptor Pico for 15 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragments. Chromatin was immunoprecipitated with a DCL1 antibody (10µg/IP) and Dynabeads®Protein G (Life Technologies). Libraries were prepared using the MicroPlex Library Preparation Kit (Diagenode) and sequenced on the NextSeq platform.
Courtesy Dr. Jakub Dolata, Laboratory of Gene Expression, UAM, Poznań, Poland
Additional information
Background
Background
DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs).
Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.
Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.
Product citations
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