DCL1 | Dicer-like protein 1
AS19 4307 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Chromatin immunoprecipitation was performed using 4 g of crosslinked (1% formaldehyde) Arabidopsis thaliana tissue as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bioruptor Pico for 15 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragments. Chromatin was immunoprecipitated with a DCL1 antibody (10µg/IP) and Dynabeads®Protein G (Life Technologies). Libraries were prepared using the MicroPlex Library Preparation Kit (Diagenode) and sequenced on the NextSeq platform.
Courtesy Dr. Jakub Dolata, Laboratory of Gene Expression, UAM, Poznań, Poland
Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.
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