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Homogalacturonan-1 (clone CCRC-M38)

AS16 3117  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

Homogalacturonan-1 (clone CCRC-M38) in the group Antibodies for Plant/Algal  / Cell Wall at Agrisera AB (Antibodies for research) (AS16 3117)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent. Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4).

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Format Cell culture supernatant, liquid.
Quantity 5 ml
Storage

Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C.

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution

1:10 (IHC)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified α-1,4 linked homogalcturonan (HG) epitope (DP>4).
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information This antibody recognises fully de-esterified α-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4). Does not recognize a homogalacturonan trimer.
This antibody binds to a de-esterified α-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more. It does not bind to a homogalacturonan trimer.

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Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.

Product citations

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