EGFP | Enhanced Green Fluorescence Protein
AS21 4583 | Clonality: Polyclonal | Host: Rabbit | Reactivity: EGFP
|Data sheet||Product citations||Add review|
Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0
Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.
Expiry date: 12 months after purchase if unopened
Left panel: Analysis of GFP expression (green) in transfected HEK293 cells by immunocytochemistry. Cells were stained with rabbit anti-GFP antibody (red, 1:2,000). Blue: Hoechst staining for nuclei. Transfected cells appear orange-yellow due to the green (GFP) and red (anti-GFP antibody) color overlay.
Right panel: Western blot analysis of GFP protein in transfected HEK293 lysate using rabbit antibody to GFP (green, 1:2,000).  protein standard,  non-transfected control cells,  transfected cells with GFP construct,  transfected cells with mCherry construct. A strong band at ~27 kDa corresponds to the expected molecular weight of GFP protein detected only in cells transfected with GFP construct. The antibody does not cross-react with mCherry protein. The same blot was simultaneously probed with a mouse antibody to beta-tubulin (red, ~ 50 kDa, lanes 2-4).
EGFP has Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000.
Related products: EGFP | Enhanced Green Fluorescence Protein
This product can b...