Donkey anti-Sheep IgG (H&L), DyLight® 550 conjugated

AS12 1983 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG


product information
Background		Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight®550 Conjugate is a secondary antibody conjugated to DyLight® 550, which binds to all Sheep IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase Sheep IgG (H&L). DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Immunogen		Purified Sheep IgG, whole molecule
Host		Donkey
Clonality		Polyclonal
Clone
Purity		Affinity purified donkey IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products		other dylight anti-sheep secondary antibodies
Additional information		Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

application information
Recommended dilution		1 : 20-1 : 2000 for most applications
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information		Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins. No reactivity is observed to: non-immunoglobulin sheep serum proteins. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Selected references		to be added when available

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