DYKDDDDK (binds to Sigma FLAGŪ) (mouse antibody, monoclonal)
AS20 4380 | Clonality: Monoclonal | Host: Mouse | Reactivity: DYKDDDDK-tag
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KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
Purity Affinity purified, in 10 mM PBS pH 7.2 with 0.05 % sodium azide
Quantity 100 µg
Storage Store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipiation (IP), Western blot (WB)
Recommended dilution 1: 500 - 1: 2000 (Dot), (ELISA), (IP), 1 : 1000 - 1: 3000 (WB)
Confirmed reactivity DYKDDDDK (Sigma FLAG®)
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Antibody detects N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins and 1-5 ng of FLAG tagged proteins in Western blot using chemiluminescent detection.
Background Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
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