ER | Estrogen receptor

AS05 065R | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

Product Information

Immunogen

KLH conjugated synthetic peptide conserved in sequences of all known ER-alpha and ER-beta including human ER-alpha PO3372 and human ER-beta Q92731

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5 000 (WB)

Expected | apparent MW

60-65 kDa

Reactivity

Confirmed reactivity Human Recombinant ER

Predicted reactivity Mammals

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example: Western blot analysis

Additional information

Additional information Antibody will recognize both isoforms: ER-alpha and ER-beta

Background

The estrogen receptor (ER) is a steroid hormone receptor. This protein functions as a nuclear receptor for estrogens such as estradiol. The two different estrogen receptor proteins produced from the ESR1 and ESR2 genes are called the alpha and beta receptors. Alternative names of the protein coded by ESR1 gene: estradiol receptor, ER-alpha, nuclear receptor subfamily 3 group A member 1. Alternative names of the protein coded by ESR2 gene: nuclear receptor subfamily 3 group A member 2.

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Confirmed reactivity:	Human Recombinant ER
predicted reactivity:	Mammals
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	The estrogen receptor (ER) is a steroid hormone receptor. This protein functions as a nuclear receptor for estrogens such as estradiol. The two different estrogen receptor proteins produced from the ESR1 and ESR2 genes are called the alpha and beta receptors. Alternative names of the protein coded by ESR1 gene: estradiol receptor, ER-alpha, nuclear receptor subfamily 3 group A member 1. Alternative names of the protein coded by ESR2 gene: nuclear receptor subfamily 3 group A member 2.
calculated \| apparent molecular mass [kDa]:	60-65 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH conjugated synthetic peptide conserved in sequences of all known ER-alpha and ER-beta including human ER-alpha PO3372 and human ER-beta Q92731
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	100 µg
recommended dilution:	1 : 5 000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Picture (footer):	application example: Western blot analysis
additional information:	Antibody will recognize both isoforms: ER-alpha and ER-beta