F7 | Coagulation factor VII

IMS01-118-307 | Clonality: Polyclonal | Host: Hen | Reactivity: Human

Product Information

Immunogen

Purified full length recombinant protein UniProt:P08709

Host Chicken

Clonality Polyclonal

Purity Total IgY

Format Liquid in 0.9% NaCl, 0.1% sodium azide

Quantity 100 µl (11 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Total, purified IgY

Recommended dilution 1 : 1000-1 : 5 000 (ELISA), 1 : 5 000-1 : 10 000 (WB)

Expected | apparent MW

52 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Primates

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

he IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

The antibody, in dilution 1: 10 000, will detect 1 ng of factor VII in immunoblotting.

Related products

AS00 001 | Anti-Coagulation factor VIIa, rabbit antibodies

IMS01-015-308 | Anti-Coagulation factor VIII, chicken antibodies

IMS01-137-306 | Anti-Coagulation factor XII, chicken antibodies

Secondary antibodies

Background

Factor VII is a 52 kDa multi-domain, single chain plasma glycoprotein synthesised in the liver and having abnormal circulating concentration of between 400 and 600 g/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, Factor Xa, Factor IXa or Factor XIIa. Factor VIIa converts Factor X to Factor Xa, which in turn converts prothrombin to thrombin.