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FBA | Fructose-bisphosphate aldolase 1 (cytoplasmic)

AS16 3848 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa
FBA | Fructose-bisphosphate aldolase 1 (cytoplasmic) in the group Antibodies for Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS16 3848)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated peptide, derived from Oryza sativa FBA protein sequence, UniProt: P17784, LOC_Os05g33380.1

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody. Prepared aliquotes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 38 | 43 kDa

Reactivity

Confirmed reactivity Oryza sativa, Triticum aestivum
Predicted reactivity Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-FBA, cytoplasmic aldolase antibodies


50-100 µg of total protein from Oryza sativa cv. 9311 seed at the maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.

Additional information

Related products

Related products AS13 2731 | Anti-FBA | Fructose-bisphosphate aldolase class 2, rabbit antibodies

AS08 294 | Anti-ALD | fructose-1,6 bisphosphate aldolase, rabbit antibodies

antibodies involved in carbohydrate metabolism

Background

Background FBA (Fructose-bisphosphate aldolase) is an enzyme involved in glycolysis and glyconeogenesis and gibberellin mediated root growth. Alternative names: cytoplasmic aldolase, cALD, gravity-specific protein GSC 233. 

Product citations

Selected references Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.

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