DISCONTINUED FER | Ferritin (plant)

Product no: AS10 674

AS10 674 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Cucumis stivus

Replaced by AS15 2898

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  • Data sheet
    • Product Info
    • Immunogen:

      KLH-conjugated synthetic peptide derived from known plant ferritin sequences including Arabidopsis thaliana: ferritin-1 (chloroplastic), UniProt: Q39101, TAIR: AT5G01600; ferritin-2(expressed in roots), UniProt: Q9SRL5,TAIR: AT3G11050, ferritin-3 (chloroplastic), UniProt: Q9LYN2, TAIR: AT3G56090; ferritin-4 (chloroplastic), UniProt: Q9S756, TAIR: AT2G40300 (sequence identity 80 %)

      Host: Rabbit
      Clonality: Polyclonal
      Purity: Serum
      Format: Lyophilized
      Quantity: 200 l
      Reconstitution: For reconstitution add 200 l of sterile water
      Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
      Tested applications: Western blot (WB)
      Recommended dilution: 1 : 3000 (WB)
      Expected | apparent MW:

      28 | 30 kDa

    • Reactivity
    • Confirmed reactivity: Arabidopsis thaliana, Cucumis sativus
      Predicted reactivity:

      Hordeum vulgare, Musa sp., Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Solanum tuberosum, Ricinus communis,Triticum aestivum, Zea mays 

      Species of your interest not listed? Contact us
      Not reactive in:

      Lemna minor, Ostreococcus tauri

    • Application Examples
    • Application example
      western blot using anti plant ferritin antibody
      10 µg of total protein from Arabidopsis thaliala whole seedlings, various cellular fractions, extracted with (see below*) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed four times for 5 min inPBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 180 seconds.
      *Following is the protein extraction buffer composition that was used to extract the total protein from the whole seedlings:50mM Tris, 4% SDS, 1% PVPP , 5% Glycerol, 1mM PMSF, 2mM Pefabloc.

      Courtesy of Anshika Jain and Erin Connolly, University of South Carolina, USA
    • Additional Information
    • Additional information (application):

      Antibody is recognizing a chloroplastic form of plant ferritin

    • Background
    • Background:

      Ferritin is the major non-toxic iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides.

    • Product Citations
    • Selected references: Kovács et al. (2016). Revisiting the iron pools in cucumber roots: identification and localization. Planta. 2016 Jul;244(1):167-79. doi: 10.1007/s00425-016-2502-x. Epub 2016 Mar 22.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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