FGF2 | Basic fibroblast growth factor

IMS01-138-313 | Clonality: Polyclonal | Host: Hen | Reactivity: Human

Product Information

Immunogen

purified full length native protein UniProt:P09038

Host Chicken

Clonality Polyclonal

Purity Total IgY fraction

Format Liquid in 0.9 % NaCl, 0.1 % sodium azide

Quantity 100 µl (13 mg/ml)

Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Total, purified IgY

Recommended dilution 1 : 1000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

18 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Bovine, Chicken, Mouse, Rat, Sheep

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information The IgY fraction is isolated by a two-step PEG precipitation. procedure followed by ammonium sulphate precipitation.

Reacts with human recombinant FGF2. No reactivity with other human serum proteins.

Background

Basic Fibroblast Growth Factor (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.