Ficain | ficin
AS09 550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)
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Product Information
Immunogen
native ficin isolated and purified from Ficus glabrata
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)
Reactivity
Confirmed reactivity
Ficus glabrata
Predicted reactivity
Ficus glabrata
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Background
Background
Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.
Product citations
Selected references
Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515
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