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Ficain | ficin

AS09 550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)

Ficain | ficin  in the group Antibodies Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 550)
Ficain | ficin



DATA SHEET IN PDF

Qty: 
248
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

native ficin isolated and purified from Ficus glabrata

Host Rabbit
Clonality Polyclonal
Purity Purified IgG in PBS.
Format Lyophilized
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ficus glabrata
Predicted reactivity Ficus glabrata
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

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Background

Background

Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.

Product citations

Selected references Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515
immunogen:

native ficin isolated and purified from Ficus glabrata

Host: Rabbit
Clonality: Polyclonal
Purity: Purified IgG in PBS.
Format: Lyophilized
Quantity: 10 mg
Reconstitution: For reconstitution add 1 ml of sterile destilled water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution: 1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)
Confirmed reactivity: Ficus glabrata
predicted reactivity: Ficus glabrata
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application): The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
background:

Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.

All references: Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515

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