Ficain | ficin

AS09 550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)

Product Information

Immunogen

native ficin isolated and purified from Ficus glabrata

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ficus glabrata

Predicted reactivity Ficus glabrata

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Related products

AS09 551 | Anti-ficain (ficin) biotinylated, rabbit antibodies

AS09 545 | Anti-PPI | papain, rabbit antibodies

AS09 546 | Anti-PPI | papain (biotinylated), rabbit antibodies

AS09 547 | Anti-PPI | papain (affinity purified), rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.

Product citations

Selected references Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515

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