Ficain | ficin

Name: Ficain | ficin
Brand: IKP
SKU: AS09 550
Price: 248 EUR
Availability: InStock

AS09 550 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)

Ficain | ficin in the group Antibodies Plant/Algal / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 550)

Product Information

Immunogen

native ficin isolated and purified from Ficus glabrata

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ficus glabrata

Predicted reactivity Ficus glabrata

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Background

Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.

Product citations

Selected references Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515

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immunogen:	native ficin isolated and purified from Ficus glabrata
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Purified IgG in PBS.
Format:	Lyophilized
Quantity:	10 mg
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)
Confirmed reactivity:	Ficus glabrata
predicted reactivity:	Ficus glabrata
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
background:	Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.
All references:	Dini et al (2021) An Extract from Ficus carica Cell Cultures Works as an Anti-Stress Ingredient for the Skin. Antioxidants. 2021; 10(4):515. https://doi.org/10.3390/antiox10040515