showForm Anti-Ficain | ficin (biotinylated) antibodies

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Ficain | ficin, Biotin conjugated

AS09 551 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ficus glabRata (fig)

Ficain | ficin, Biotin conjugated in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 551)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

native ficin isolated and purified from Ficus glabrata

Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 ml
Reconstitution For reconstitution add 0.5 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 1000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ficus glabrata
Predicted reactivity Ficus glabrata
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

Related products

AS09 550 | anti-ficain (ficin)

AS09 545 | anti-PPI | papain

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

Plant protein extraction buffer

Background

Background

Ficin known also as ficain (EC=3.4.4.12) is a plant protease which enzymatic activity depends upon cysteine residue (cysteine endopeptidase).It is used to differentiate blood antigens. Alternative names: debricin, higueroxyl delabarre.

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