GAD67 (14-122) | Glutamic Acid Decarboxylase

AS16 3840 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Rat

Product Information

Immunogen E.coli-derived human GAD67 recombinant protein (14-122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67. UniProt: Q99259 , DCE1_HUMAN.

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage After reconstitution, centrifuge to remove any insoluble material. Storage: At -20°C for one year (lyophilized). After reconstitution, the antibody can be stored at 4°C for one month or alternatively, aliquot the vial into smaller volumes and store at -20°C for a higher stability. Avoid repeated freezing and thawing. Shelf life time is 12 months.

Tested applications Western blot (WB)

Recommended dilution 0,1-0,5 µg/ml

Expected | apparent MW

67 kDa

Reactivity

Confirmed reactivity Human, Rat

Predicted reactivity Mouse

Application examples

Additional information

The detection limit for GAD67 is approximately 0,25 ng/lane under reducing conditions

Background

Glutamic Acid Decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65. Alternative names: GAD; GAD-67; GAD1; Glutamate decarboxylase 1;

Product citations

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Picture (footer):
background:	Glutamic Acid Decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65. Alternative names: GAD; GAD-67; GAD1; Glutamate decarboxylase 1;
calculated \| apparent molecular mass [kDa]:	67 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	E.coli-derived human GAD67 recombinant protein (14-122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67. UniProt: Q99259 , DCE1_HUMAN.
Purity:	Immunogen affinity purified serum in 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide.
Quantity:	100 µg
recommended dilution:	0,1-0,5 µg/ml
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	After reconstitution, centrifuge to remove any insoluble material. Storage: At -20°C for one year (lyophilized). After reconstitution, the antibody can be stored at 4°C for one month or alternatively, aliquot the vial into smaller volumes and store at -20°C for a higher stability. Avoid repeated freezing and thawing. Shelf life time is 12 months.
tested applications:	Western blot (WB)
additional information (application):	The detection limit for GAD67 is approximately 0,25 ng/lane under reducing conditions
Confirmed reactivity:	Human, Rat
predicted reactivity:	Mouse