GFP | Green Flourescent Protein (monoclonal, clone GF28R)
AS20 4460 | Clonality: Monoclonal | Host: Mouse | Reactivity: GFP

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Product Information
Immunogen
KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Protein A/G purified
Format
Liquid in 10mM PBS, pH 7.2, with 0.05% sodium azide.
Quantity
100 µg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications
Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Recommended dilution
1: 500 - 2000 (IHC), 1:1000-3000 (WB)
Reactivity
Confirmed reactivity
Native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Application examples
Additional information
Background
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product citations
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