GFP | Green Flourescent Protein (monoclonal, clone GF28R)
AS20 4460 | Clonality: Monoclonal | Host: Mouse | Reactivity: GFP
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Immunogen KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria
Purity Protein A/G purified
Format Liquid in 10mM PBS, pH 7.2, with 0.05% sodium azide.
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Recommended dilution 1: 500 - 2000 (IHC), 1:1000-3000 (WB)
Confirmed reactivity Native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Background GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
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