GFP | Green Flourescent Protein (monoclonal, clone GF28R)

AS20 4460 | Clonality: Monoclonal | Host: Mouse | Reactivity: GFP

Product Information

Immunogen KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Protein A/G purified

Format Liquid in 10mM PBS, pH 7.2, with 0.05% sodium azide.

Quantity 100 µg

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

Recommended dilution 1: 500 - 2000 (IHC), 1:1000-3000 (WB)

Reactivity

Confirmed reactivity Native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Application examples

Additional information

Related products

Related products AS15 2998 | Anti-GFP | Green Fluorescence Protein (total IgY), chicken antibody
AS15 2987 | Anti-GFP (affinity purified), rabbit antibody
AS15 3000 | Anti-GFP (protein A purified), rabbit antibody
AS15 2999 | Anti-GFP (total IgG), rabbit antibody
AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibody
AS15 3002 | GFP (purified recombinant protein)
Other GFP-tag antibodies.
Other tag antibodies.

Background

Background GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.