GFP | Green Fluorescence Protein (total IgG)

AS15 2999 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

Product Information

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit

Clonality Polyclonal

Purity Total IgG in 10 mM TRIS, pH 7.2 with 0.02 % sodium azide

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E.coli), all variants of GFP

Application examples

Additional information

Minimal cross-reactivity with E.coli proteins

Related products

AS15 2987 | Anti-GFP (affinity purified), rabbit antibodies

AS15 3000 | Anti-GFP (protein A purified), rabbit antibodies

AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodies

AS15 2998 | Anti-GFP (total IgY), chicken antibodíes

AS15 3002 | GFP (purified recombinant protein)

other tag antibodies

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

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