GFP | Green Fluorescence Protein (total IgG)
AS15 2999 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP
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Product Information
Immunogen
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG in 10 mM TRIS, pH 7.2. Contains 0.02 % sodium azide.
Format
Lyophilized
Quantity
100 µg
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 5000-1 : 25 000 (ELISA), 1: 1000 (IF), 1 : 2000-1 : 10 000 (WB)
Reactivity
Confirmed reactivity
Native GFP, Recombinant GFP (E.coli), variants of GFP
Application examples
Additional information
Low cross-reactivity with E.coli proteins
Background
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product citations
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