GFP | Green Fluorescence Protein (protein A purified)

AS15 3000 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

Product Information

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified.

Format Liquid

Quantity 100 µg at 1 mg/ml

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP

Application examples

Application examples Application example

IF using anti/GFP | Green Fluorescence Protein polyclonal antibodies

IF using anti-GFP | Green Fluorescence Protein antibodies

EGFP-tagged nuclear lamins (in green), detected in the same cell by anti-GFP | Green Fluorescence Protein antibodies (in red; dilution 1:500).

Mouse fibroblasts were transfected with an EGFP-Lamin A hybrid construct and indirectly immunostained using anti-GFP | Green Fluorescence Protein as the primary antibody. Fixation and permeabilization was performed with 4% buffered formaldehyde for 10 min. at room temperature and treatment with 0.1% Triton X-100 for 10 min., respectively.

Additional information

Minimal cross-reactivity with E.coli proteins

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

Selected references Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.

All references:

Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.

additional information (application):

Minimal cross-reactivity with E.coli proteins

background:

Clonality:

Polyclonal

Format:

Liquid

Host:

Rabbit

immunogen:

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Purity:

Total IgG. Protein A purified.

Quantity:

100 µg at 1 mg/ml

recommended dilution:

1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

storage:

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

tested applications:

ELISA (ELISA), Western blot (WB)

Picture (footer):

Application example

Confirmed reactivity:

Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP