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GFP | Green Fluorescence Protein (protein A purified)

AS15 3000 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

GFP | Green Fluorescence Protein (protein A purified) in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS15 3000)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit
Clonality Polyclonal
Purity protein A purified
Format

Liquid

Quantity 100 µg at 1mg/ml
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP

Application examples

Application examples Application example

IF using anti/GFP | Green Fluorescence Protein polyclonal antibodies IF using anti-GFP | Green Fluorescence Protein antibodies


EGFP-tagged nuclear lamins (in green), detected in the same cell by anti-GFP | Green Fluorescence Protein antibodies (in red; dilution 1:500).

Mouse fibroblasts were transfected with an EGFP-Lamin A hybrid construct and indirectly immunostained using anti-GFP | Green Fluorescence Protein as the primary antibody. Fixation and permeabilization was performed with 4% buffered formaldehyde for 10 min. at room temperature and treatment with 0.1% Triton X-100 for 10 min., respectively.

Additional information

Minimal cross-reactivity with E.coli proteins

Related products

Related products

AS15 2987 | Anti-GFP (affinity purified), rabbit antibodies

AS15 2999 | Anti-GFP (total IgG), rabbit antibodies

AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodies

AS15 2998 | Anti-GFP (total IgY), chicken antibodies

AS15 3002 | GFP (purified recombinant protein)


other tag antibodies

Background

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

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