GFP | Green Fluorescence Protein (protein A purified)
AS15 3000 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP
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highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
EGFP-tagged nuclear lamins (in green), detected in the same cell by anti-GFP | Green Fluorescence Protein antibodies (in red; dilution 1:500).
Mouse fibroblasts were transfected with an EGFP-Lamin A hybrid construct and indirectly immunostained using anti-GFP | Green Fluorescence Protein as the primary antibody. Fixation and permeabilization was performed with 4% buffered formaldehyde for 10 min. at room temperature and treatment with 0.1% Triton X-100 for 10 min., respectively.
Minimal cross-reactivity with E.coli proteins
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.