GFP | Green Fluorescent Protein (monoclonal)

AS18 4175 | Clonality: Monoclonal | Host: Mouse | Reactivity: GFP

Product Information

Immunogen KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria P42212.

Host Mouse

Clonality Monoclonal

Purity Affinity purified in PBS, pH 7.4 with 0.05% sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at -20°C. Store in small aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Dot Blot, Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)

Recommended dilution 1:1000 (WB)

Reactivity

Confirmed reactivity Native and denatured forms of GFP and Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).

Application examples

Application examples Application example

Western Blot analysis of mouse monoclonal anti-GFP tag. Negative control (1), GFP fusion protein positive 293 cells (10µg lysate) with 1µg of anti-GFP tag antibody (2). GFP fusion protein positive 293 cells (10µg lysate) with 10µg of anti-GFP tag antibody (3).

Additional information

Related products

Related products AS15 2998 | Anti-GFP | Green Fluorescence Protein (total IgY), chicken antibody
AS15 2987 | Anti-GFP (affinity purified), rabbit antibody
AS15 3000 | Anti-GFP (protein A purified), rabbit antibody
AS15 2999 | Anti-GFP (total IgG), rabbit antibody
AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibody
AS15 3002 | GFP (purified recombinant protein) Other GFP-tag antibodies.
Other tag antibodies.

Background

Background GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.