GFP | Green Fluorescent Protein (monoclonal)
AS18 4175 | Clonality: Monoclonal | Host: Mouse | Reactivity: GFP

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Product Information
Immunogen
KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria P42212.
Host
Mouse
Clonality
Monoclonal
Purity
Affinity purified in PBS, pH 7.4 with 0.05% sodium azide.
Format
Liquid
Quantity
100 µg
Storage
Store at -20°C. Store in small aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Dot Blot, Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
Recommended dilution
1:1000 (WB)
Reactivity
Confirmed reactivity
Native and denatured forms of GFP and Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).
Application examples
Application examples
Application example

Western Blot analysis of mouse monoclonal anti-GFP tag. Negative control (1), GFP fusion protein positive 293 cells (10µg lysate) with 1µg of anti-GFP tag antibody (2). GFP fusion protein positive 293 cells (10µg lysate) with 10µg of anti-GFP tag antibody (3).

Western Blot analysis of mouse monoclonal anti-GFP tag. Negative control (1), GFP fusion protein positive 293 cells (10µg lysate) with 1µg of anti-GFP tag antibody (2). GFP fusion protein positive 293 cells (10µg lysate) with 10µg of anti-GFP tag antibody (3).
Additional information
Background
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product citations
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